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1. Xcalibur software, free download
2. Xcalibur software, free download

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You can use the instrument control software to collect high-quality mass spectrometry data on the Thermo Scientific mass spectrometers. Control of the instruments is through two application packages: Tune and Method Editor.

For questions about the software, to request features, or to report defects, please email planet.orbitrap@thermofisher.com.

Release date: September 22, 2020
Build number: 3.4.3072.18
Instruments: Orbitrap Eclipse MS, Orbitrap Fusion Lumos MS, Orbitrap Fusion MS, and Orbitrap ID-X MS

Software requirements: Xcalibur 4.3 or later (use 4.4 for the latest AcquireX features). Note, use of Orbitrap Tribrid Series 3.4 requires Windows 10. Please see the Update Overview for Windows 10 PC information.

Follow the upgrade instructions provided:Orbitrap Tribrid MS Series ICSW v. 3.4 Release Notes

Download the software:Orbitrap Tribrid MS Series ICSW v. 3.4 and Xcalibur 4.4 Software
Download the Update Overview: Orbitrap Tribrid MS Series ICSW v.3.4 Overview Play roulette for free.

New Features

The Orbitrap Tribrid MS Series instrument control software version 3.4 incorporates the following new and improved features:

• (Orbitrap Eclipse only) Support for Real-Time Search with multiple FAIMS CVs, neutral loss option, 'closeout' option, and FDR-based filtering
• (Orbitrap Eclipse only) Support for PTCR with tMS3 experiments
• RunStart EASY-IC mass calibration performs internal mass correction for each selected run without having to perform EASY-IC per individual spectrum
• Time dependent gas mode provides flexibility for gas settings during the method
• Isotope exclusion filter permits isotope exclusion without having to use the dynamic exclusion filter
• 'Block Trigger' option lets the user prevent dependent scans from triggering when specific ions or ion patterns are detected (that is, opposite of standard trigger function)
• Full MS microscans are acquired in parallel during method runs
• Stepped collision energy parameter allows 2 to 5 collision energy levels for HCD instead of requiring 3
• Dynamic Exclusion filter supports use of a shared exclusion list that can reduce redundant triggering between experiments (that is, in the case of a FAIMS multi-CV method)
• MIPS filter can be placed selectively per outcome, when multiple dependent scans are present
• (Intact Protein mode only) Automatic adjustment of the dynamic exclusion tolerances based upon whether the precursor ion was annotated with the low resolution and high resolution APD algorithm
• Usability enhancements:
  • Support for auto-calibration including a new 'Check, calibrate if required' mode, which automates the expert user behavior for quickly checking calibrations and calibrating only those items needed, which are based on the time passed since the previous calibration or Pass/Fail status.
  • Support for iAPI agreement and license access directly through the Tune application interface
  • Support for automated service team notification from Almanac, when acute system issues occur
  • Support for corrective action notifications from Almanac
  • Support for web-based method template updates
  • Improved default settings for small molecule application mode

Known Issues

• When using Windows 10, the View Raw File button does not open the raw file properly, nor can you double-click the raw file to open it.
  • Instead, navigate to the raw file, right-click it, and browse to the appropriate application (FreeStyle) to open the file. Select the checkbox to always open a raw file with the selected application, which then becomes the default. (Reported in Release Notes 3.0 and 3.1)
• A patch is available here to fix a communication error in software version 3.4 associated with the UVPD laser controller, leading to a CLOL fault.
  • Instructions to fix this issue are available here.
• A patch is available here to fix an issue in software version 3.4 associated with the coarse calibration adjustment.
  • Instructions to fix this issue are available here.
• A patch is available here to works around a problem in software version 3.4 in which split peaks could be observed only after instrument bootup.
  • Instructions to fix this issue are available here.

Instructions to fix this issue are available here.

• Registerfor Orbitrap Tribrid Series MS Technical updates and to receive alerts about newly discovered defects.

Fixed Defects

• See Orbitrap Tribrid MS Series ICSW v. 3.4 Release Notes

Contact Us

• Request a new feature
• Report a software issue

Release date: October 26, 2020
Instruments: Orbitrap Exploris 480, Orbitrap Exploris 240, and Orbitrap Exploris 120

Software requirements: Xcalibur 4.4

Follow the upgrade instructions provided:Orbitrap Exploris Series Instrument Control Software vs. 2.0 SP2 Release Notes

Download the software:Orbitrap Exploris MS Series ICSW v. 2.0 SP2 and Xcalibur 4.4 Software

New Features

The Orbitrap Exploris Series instrument control software version 2.0 SP2 incorporates the following new and improved features from version 1.1 SP1:

• Orbitrap Exploris 120 Method Editor: Extend Data Dependent MS2 Method to create TopN experiments with N = 1, 2, 3, or 4
• Orbitrap Exploris 120 Method Editor: Provide System Templates for TopN experiments with N = 1, 2, 3, or 4
• BioPharma: AHFP mass calibration in Tune Diagnostics
• AcquireX: Orbitrap Exploris 240 and 480 support AcquireX workflows for Application Mode 'Small molecule'
• iAPI provides access to real-time control of Orbitrap Exploris 480
• Method Editor provides access to latest System Templates updates directly via the Thermo Fisher Cloud
• Method Editor: Application Mode ‘Small Molecule' provides optionally access to Mild Trapping settings to reduce MS1 fragmentation of labile compounds
• Method Editor: Ion Source pane provides access to time-dependent ion source gas flows
• Method Editor: RunStart EASY-IC allows for on-the-fly mass correction using an automated EASY-IC lock-mass correction factor
• Method Editor: tMS2 and DIA scans use isochronous injection times for multiplexing experiments
• Method transfer: Method Editor is able to open and save methods from all Orbitrap Exploris Series instruments
• Tune Calibration pane: System Calibration provides improved help text in gray box, Alert Window and Calibration Reports
• Chromeleon: Orbitrap Exploris Series 2.0 runs under Chromeleon 7.2.10 and 7.3
• FAIMS Pro provides same optimized CV values aligned with Tribrid Series and TSQ Series instruments
• Method Editor: DIA Scan AGC target is aligned with Tribrid Series - Normalized Base (100%) is equal to 1e5
• Method Editor: Mass List Tables: MRFA is default compound aligned with Tribrid Series
• Method Editor: Mass List Tables: Pop-up Warning 'Columns don't match' has improved messages when importing Exactive Series Global Lists
• Method Editor: Targeted MS2 Scans: 'Dynamic RT' provides a Retention Time Standards Table for Pierce PRTC Mixture (Orbitrap Exploris 240 and Orbitrap Exploris 480 models)
• Scan Filter: Methods using Internal Mass Calibration hide 'lock' to improve generating XICs from the same scan filter
• Tune Calibration pane: Recommended Date of a mass calibration updates after successful system calibration
• Tune: Ion Source pane provides improved default Ion Source Parameters

Known Issues

• Registerfor Orbitrap Exploris Series MS Technical updates and to receive alerts about newly discovered defects.

• See Orbitrap Tribrid MS Series ICSW v. 3.4 Release Notes

Contact Us

• Request a new feature
• Report a software issue

Release date: October 26, 2020
Instruments: Orbitrap Exploris 480, Orbitrap Exploris 240, and Orbitrap Exploris 120

Software requirements: Xcalibur 4.4

Follow the upgrade instructions provided:Orbitrap Exploris Series Instrument Control Software vs. 2.0 SP2 Release Notes

Download the software:Orbitrap Exploris MS Series ICSW v. 2.0 SP2 and Xcalibur 4.4 Software

New Features

The Orbitrap Exploris Series instrument control software version 2.0 SP2 incorporates the following new and improved features from version 1.1 SP1:

• Orbitrap Exploris 120 Method Editor: Extend Data Dependent MS2 Method to create TopN experiments with N = 1, 2, 3, or 4
• Orbitrap Exploris 120 Method Editor: Provide System Templates for TopN experiments with N = 1, 2, 3, or 4
• BioPharma: AHFP mass calibration in Tune Diagnostics
• AcquireX: Orbitrap Exploris 240 and 480 support AcquireX workflows for Application Mode 'Small molecule'
• iAPI provides access to real-time control of Orbitrap Exploris 480
• Method Editor provides access to latest System Templates updates directly via the Thermo Fisher Cloud
• Method Editor: Application Mode ‘Small Molecule' provides optionally access to Mild Trapping settings to reduce MS1 fragmentation of labile compounds
• Method Editor: Ion Source pane provides access to time-dependent ion source gas flows
• Method Editor: RunStart EASY-IC allows for on-the-fly mass correction using an automated EASY-IC lock-mass correction factor
• Method Editor: tMS2 and DIA scans use isochronous injection times for multiplexing experiments
• Method transfer: Method Editor is able to open and save methods from all Orbitrap Exploris Series instruments
• Tune Calibration pane: System Calibration provides improved help text in gray box, Alert Window and Calibration Reports
• Chromeleon: Orbitrap Exploris Series 2.0 runs under Chromeleon 7.2.10 and 7.3
• FAIMS Pro provides same optimized CV values aligned with Tribrid Series and TSQ Series instruments
• Method Editor: DIA Scan AGC target is aligned with Tribrid Series - Normalized Base (100%) is equal to 1e5
• Method Editor: Mass List Tables: MRFA is default compound aligned with Tribrid Series
• Method Editor: Mass List Tables: Pop-up Warning 'Columns don't match' has improved messages when importing Exactive Series Global Lists
• Method Editor: Targeted MS2 Scans: 'Dynamic RT' provides a Retention Time Standards Table for Pierce PRTC Mixture (Orbitrap Exploris 240 and Orbitrap Exploris 480 models)
• Scan Filter: Methods using Internal Mass Calibration hide 'lock' to improve generating XICs from the same scan filter
• Tune Calibration pane: Recommended Date of a mass calibration updates after successful system calibration
• Tune: Ion Source pane provides improved default Ion Source Parameters

Known Issues

• Registerfor Orbitrap Exploris Series MS Technical updates and to receive alerts about newly discovered defects.

Fixed Defects

• See Orbitrap Exploris Series Instrument Control Software vs. 2.0 SP2 Release Notes

Contact Us

• Request a new feature
• Report a software issue

New Features

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The Exactive Series instrument control software version 2.9 SP2 incorporates the following new and improved features from Exactive Series 2.9:

Xcalibur software, free download

• QE Plus, QE HF, QE HF-X:
  • Evaluation of given mass calibration for HMR in negative ion mode
  • Calibration of negative ion mode when HMR templates are used
  • The advanced user must have a robust C-Trap Exit Lens–Purge Optimization
• QE Plus, QE HF, QE HF-X: Method Editor:
  • Capable of applying negative ion mode/polarity when HMR Full MS template applied
  • Capable of applying negative ion mode/polarity when HMR AIF template is applied
• GC Q Exactive, GC Exactive:
  • HCD Collision Energy Limit
  • Enable access to enhanced speed mode, 7.5 k resolution
• 2.11 QF1 (available for download only) fixes a defect of not showing ‘Isolation Transmission Endurance Check' to user role ‘Advanced'.

Known Issues

• For more details, defect fixes and known issues, please refer to the Release Notes of 2.11 and 2.11 QF1.

Fixed Defects

• See Exactive Series Instrument Control Software vs. 2.11 QF1 Release Notes

Xcalibur software, free download

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